Antibody Screening

VERAXA constantly invests considerable resources in the competitive expansion of its know-how in biological assay development for functional assessment of highly relevant complex targets such as membrane receptors (like GPCRs) or life-threatening pathogens. Due to its additional strong roots in technological development, opto-electrical hardware design, software conception and machine integration, VERAXA offers an unmet compilation of screening and assay tools, paired with superior high performance machine automation.

Functional Antibodies

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Anti-Pathogenic Antibodies

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Personalized Antibodies

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Currently, our proprietary »Hitmaster« workstation represents leading benchmark characteristics in:

  • functionality – multiplexed 4 color detection and real time quantitative analysis and sorting,
  • robustness – optimized and shortest optical paths, leading laser hub technology
  • system integration – small functional units, embedded software concept.

Over the last years, VERAXA has developed a superior toolbox for screening and development of:

  • Functional antibodies, are antibodies that exert a modulatory function – either anta- or agonizing – on complex target proteins. Here, VERAXA offers strong assay systems and expertise in assessing anti-GPCR modulating antibodies.
  • Anti-pathogenic antibodies are antibodies that kill or inhibit life threatening bacteria or viruses.

In general, antibodies can interact in different ways with target membrane proteins on reporter cells: they can bind to target receptors or ligands of interest, but only a tiny portion of the binders also induce a modulation of receptor signaling.

VERAXA screens and sorts out only those binders, that have a target receptor associated function on its intra-cellular signaling cascade, either agonistic or antagonistic. Using our unique proprietary and peer reviewed ‘function-first’ technology, we directly measure and quantify reporter cell signals induced by modulating functional antibodies in one single step. By exclusion of target-binding, but non-functional antibodies early on, the number of candidates for characterization and further engineering can be focused on high-potentials, thus reducing time and effort of lead candidate identification.

How antibodies interact with target cells

There are several ways, in which antibodies may interact with target cells

Function Up

Functional antibodies may agonize the receptor of interest and increase the related intracellular signaling. During screening, the strength of the intracellular receptor-antibody mediated signal is quantitatively measured in real time and serves as first pass sorting filter.

Functional upregulation could also be caused by antibodies, that act as allosteric enhancers. Here, the binding to membrane receptors induces a conformational change of the target receptor, thus enabling binding of the cognate ligand with greater affinity.

Function Down

The same procedure holds for antagonizing antibodies, i.e. antibodies that may decrease receptor mediated intracellular signal cascades by binding to the receptors and blocking the natural epitope for ligand docking.

Functional receptor antagonism may also be induced too by antibodies, that bind to and thus block receptor ligands from their interaction with the receptor.

VERAXA has set up procedures and assays to analyze the relevant fluorescent readout of the intracellular signal cascades within droplets housing a competition mix of B-cell, receptor cell and natural ligand.

No Function

Antibodies can bind to their complementary receptor structure without modulating any intracellular receptor signal cascade (non-functional mAb). Those non-functional binders may however still be inducers of Fc-mediated effector function.